1,845
edits
Changes
no edit summary
NOTE: The objective of this first exercise is merely to gain an awareness of the varieties of cell sizes, cell shapes, cell types, cell staining characteristics and cell organelles or inclusions. You are not expected, at this time, to become familiar with the over-all structure of the tissues and organs where these cells are located.
=== Microscopic Study of Slides: Architecture ===
==== Slide 25: Spinal Cord ====
On slide 25, Spinal Cord (Thionin) find under low power the cell bodies of multipolar neurons located in the two anterior horns of the gray matter (if the slide is held towards the light, the gray matter appears H-shaped). With medium power, identify a cell body containing a large pale nucleus and a darkly stained nucleolus. Study this cell under high power. The irregular, granular-like, basophilic staining masses within the cytoplasm are called Nissl bodies. They consist of free ribosomes and granular endoplasmic reticulum.
<peir-vm>UAB-Histology-00154</peir-vm>
=== Microscopic Study of Slides: Cell Types ===
==== Slide 2: Trachea ====
On slide 2, Trachea (H &E) identify the cilia on the tall cells of the pseudostratified columnar epithelium that line the lumen of the trachea. Each cilium is derived from a basal body, represented here in aggregate by the dark lines where the cilia attach to the cell. In some regions of this tissue the cilia are absent or the entire epithelium is missing. This is artifact.
<peir-vm>UAB-Histology-00031</peir-vm>
== Mitosis ==
Mitosis can be viewed as the means whereby identical genetic material, contained in the chromosomes, is distributed to two daughter nuclei. It can be divided into four stages, each characterized by certain features of nuclear or chromosome morphology and chromosome movement. The stages are arbitrary in that mitosis is a continuous process from its inception at prophase through the stages of metaphase and anaphase to the final stage of telophase. The nucleus of a cell that is not dividing is in the interphase stage.
The number of mitotic figures in a tissue is an index of the rate of turnover of the component cells. In benign tumors, mitotic figures are few in numbers, whereas in malignant tumors, mitotic figures are more numerous and may include many bizarre forms. Hence, the recognition of mitotic figures is one criterion for the interpretation of various kinds of pathology of a tissue.
=== Microscopic Study: Mitosis ===
On slide 34, Mitosis (Iron H), are longitudinal sections of onion root tips in which cells have been fixed in various stages of mitosis. Learn to identify the characteristic arrangement of the chromatin in each state.
<peir-vm>UAB-Histology-00034</peir-vm>
{{Template:Histologic}}
[[Category:Histologic]]
